2W2H
Structural basis of transcription activation by the Cyclin T1-Tat-TAR RNA complex from EIAV
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-06-30 |
| Detector | ADSC CCD |
| Spacegroup name | I 4 |
| Unit cell lengths | 149.460, 149.460, 129.750 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.927 - 3.250 |
| R-factor | 0.2451 |
| Rwork | 0.243 |
| R-free | 0.27840 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2pk2 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 2.211 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.250 |
| High resolution limit [Å] | 3.250 | 3.200 |
| Rmerge | 0.140 | 0.950 |
| Number of reflections | 22461 | |
| <I/σ(I)> | 8.74 | 8.74 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 5 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.4 | 7% PEG 8000, 0.1M HEPES PH7.4 0.1 M AMMONIUM SULPHATE, 15 MM MNCL2, 5% ETHYLENE GLYCOL |
