2W1A
Non-covalent complex between dahp synthase and chorismate mutase from Mycobacterium tuberculosis with bound tsa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-3 |
| Synchrotron site | ESRF |
| Beamline | ID14-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-05-17 |
| Detector | ADSC CCD |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 205.910, 205.910, 67.240 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.840 - 2.350 |
| R-factor | 0.19252 |
| Rwork | 0.191 |
| R-free | 0.22918 |
| Structure solution method | OTHER |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.157 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.860 | 2.480 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.100 | 0.450 |
| Number of reflections | 67275 | |
| <I/σ(I)> | 9.85 | 2.7 |
| Completeness [%] | 98.9 | 95.4 |
| Redundancy | 3.62 | 3.25 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.9 | 0.9M AMMONIUM SULFATE, 0.1M TRIS PH 7.9, 5% PEG400 |
