2W0F
Potassium Channel KcsA-Fab Complex with Tetraoctylammonium
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-11-19 |
Detector | MARRESEARCH |
Spacegroup name | I 4 |
Unit cell lengths | 155.760, 155.760, 75.889 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.400 |
R-factor | 0.204 |
Rwork | 0.200 |
R-free | 0.24100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1K4C WITH SELECTIVITY FILTER AND IONS REMOVED |
RMSD bond length | 0.010 |
RMSD bond angle | 1.260 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.540 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.040 | 0.480 |
Number of reflections | 35130 | |
<I/σ(I)> | 20.6 | 2.5 |
Completeness [%] | 97.8 | 96.3 |
Redundancy | 3.24 | 3.27 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5.4 | 18-25% PEG400, 50 MM SODIUM ACETATE, 50 MM MAGNESIUM ACETATE, pH 5.4 |