2VYP
Rabbit-muscle G-actin in complex with myxobacterial rhizopodin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X12 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X12 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-05-02 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 77.653, 194.863, 53.038 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.410 - 2.350 |
R-factor | 0.189 |
Rwork | 0.186 |
R-free | 0.24100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1j6z |
RMSD bond length | 0.017 |
RMSD bond angle | 1.709 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.500 |
High resolution limit [Å] | 2.350 | 2.350 |
Rmerge | 0.050 | 0.550 |
Number of reflections | 985534 | |
<I/σ(I)> | 28.4 | 3.5 |
Completeness [%] | 99.8 | 99.5 |
Redundancy | 8 | 7.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.6 | 100 MM MES PH 6.6 14% (W/W) PEG1500 12% (W/W) 1,6-HEXANEDIOL 100 MM CACL2 1 MM DTT 10 MM BETAINE-HYDROCHLORIDE |