2VXY
The structure of FTsZ from Bacillus subtilis at 1.7A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX14.1 |
| Synchrotron site | SRS |
| Beamline | PX14.1 |
| Temperature [K] | 287 |
| Detector technology | CCD |
| Collection date | 2005-05-03 |
| Detector | ADSC CCD |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 87.170, 87.170, 89.460 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 36.000 - 1.700 |
| R-factor | 0.186 |
| Rwork | 0.184 |
| R-free | 0.21800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fsz |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.461 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.770 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.100 | 0.610 |
| Number of reflections | 43398 | |
| <I/σ(I)> | 14.8 | 1.9 |
| Completeness [%] | 99.3 | 86.6 |
| Redundancy | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | pH 8.0 |
