2VX3
Crystal structure of the human dual specificity tyrosine- phosphorylation-regulated kinase 1A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-05-09 |
Detector | MARRESEARCH |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 264.203, 65.105, 140.282 |
Unit cell angles | 90.00, 115.44, 90.00 |
Refinement procedure
Resolution | 26.000 - 2.400 |
R-factor | 0.186 |
Rwork | 0.185 |
R-free | 0.23000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1EXE AND 1Z57 |
RMSD bond length | 0.014 |
RMSD bond angle | 1.518 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.4.0066) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 26.350 | 2.530 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.080 | 0.680 |
Number of reflections | 92815 | |
<I/σ(I)> | 10.2 | 2 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 3.5 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8.5 | 34% PEG 300, 0.1 M LISO4, TRIS PH 8.5 |