2VWB
Structure of the archaeal Kae1-Bud32 fusion protein MJ1130: a model for the eukaryotic EKC-KEOPS subcomplex involved in transcription and telomere homeostasis.
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-03-20 |
Detector | ADSC CCD |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 147.418, 148.948, 65.118 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.950 - 3.050 |
R-factor | 0.224 |
Rwork | 0.220 |
R-free | 0.30100 |
Structure solution method | SAD |
Starting model (for MR) | 1ivn |
RMSD bond length | 0.011 |
RMSD bond angle | 1.478 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SHARP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 3.210 |
High resolution limit [Å] | 3.050 | 3.050 |
Rmerge | 0.100 | 0.560 |
Number of reflections | 26424 | |
<I/σ(I)> | 12.4 | 2.8 |
Completeness [%] | 94.7 | 96.2 |
Redundancy | 5 | 5.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5.6 | 0.1 M SODIUM CITRATE, 5-10% ISOPROPANOL, 0.1 M SODIUM CITRATE PH 5.6 |