2VVR
Crystal structure of the H99N mutant of ribose-5-phosphate isomerase B from E. coli soaked with ribose 5-phosphate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2007-03-05 |
Detector | ADSC CCD |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 89.701, 51.862, 207.209 |
Unit cell angles | 90.00, 115.55, 90.00 |
Refinement procedure
Resolution | 40.000 - 2.100 |
R-factor | 0.19 |
Rwork | 0.188 |
R-free | 0.23700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1nn4 |
RMSD bond length | 0.012 |
RMSD bond angle | 1.175 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | AMoRE |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.490 | 2.210 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.110 | 0.170 |
Number of reflections | 50635 | |
<I/σ(I)> | 14.1 | 5.8 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 3.6 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 20% PEG 3350, 0.1 M MES PH6 |