2VVL
The structure of MAO-N-D3, a variant of monoamine oxidase from Aspergillus niger.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-09-14 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 103.287, 187.248, 132.432 |
| Unit cell angles | 90.00, 90.10, 90.00 |
Refinement procedure
| Resolution | 132.450 - 2.450 |
| R-factor | 0.185 |
| Rwork | 0.182 |
| R-free | 0.23600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | SELENOMET STRUCTURE OF MONOAMINE OXIDASE |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.515 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.4.0065) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.540 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Rmerge | 0.050 | 0.160 |
| Number of reflections | 184776 | |
| <I/σ(I)> | 15 | 5.2 |
| Completeness [%] | 97.1 | 90.8 |
| Redundancy | 2.1 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 20% PEG 3350, 0.2M AMMONIUM SULPHATE, 0.1M HEPES PH 7.5 |
