2VUQ
Crystal structure of a human tRNAGly acceptor stem microhelix (derived from the gene sequence DG9990) at 1.18 Angstroem resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-04-21 |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 37.318, 37.613, 30.465 |
| Unit cell angles | 90.00, 112.60, 90.00 |
Refinement procedure
| Resolution | 22.250 - 1.180 |
| R-factor | 0.2052 |
| Rwork | 0.204 |
| R-free | 0.22806 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2v7r |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.627 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.220 |
| High resolution limit [Å] | 1.180 | 1.180 |
| Rmerge | 0.080 | 0.210 |
| Number of reflections | 12058 | |
| <I/σ(I)> | 22.9 | 1.8 |
| Completeness [%] | 94.0 | 87 |
| Redundancy | 11.1 | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 294 | 10% (V/V) MPD, 40 MM SODIUM CACODYLATE, PH 6.0, 12 MM SPERMINE TETRACHLORIDE, 80 MM POTASSIUM CHLORIDE, EQUILIBRATED AGAINST 35 % (V/V) MPD AT 294 K. |
