2V7R

Crystal structure of a human tRNAGly microhelix at 1.2 Angstrom resolution

Summary for 2V7R

• Entry DOI: 10.2210/pdb2v7r/pdb
• Descriptor: HUMAN TRNAGLY MICROHELIX (3 entities in total)
• Functional Keywords: trna, acceptor stem, glycine, microhelix, identity, hydration, rna
• Biological source: HOMO SAPIENS (HUMAN); More
• Total number of polymer chains: 2
• Total formula weight: 4432.77

Authors

Foerster, C.,Mankowska, M.,Fuerste, J.P.,Perbandt, M.,Betzel, C.,Erdmann, V.A. (deposition date: 2007-08-01, release date: 2008-03-18, Last modification date: 2024-05-08)

Primary citation

Foerster, C.,Mankowska, M.,Fuerste, J.P.,Perbandt, M.,Betzel, C.,Erdmann, V.A.
Crystal Structure of a Human Trnagly Microhelix at 1.2 A Resolution.
Biochem.Biophys.Res.Commun., 368:996-, 2008

PubMed Abstract: The tRNA(Gly)/glycyl-tRNA synthetase (GlyRS) system belongs to the so-called 'class II aminoacyl-tRNA synthetase system' in which tRNA identity elements are assured by rather few and simple determinants mostly located in the tRNA acceptor stem. Regarding evolutionary aspects, the tRNA(Gly)/GlyRS system is a special case. There exist two different types of GlyRS, namely an archaebacterial/human type and a eubacterial type reflecting an evolutionary divergence within this system. Here we report the crystal structure of a human tRNA(Gly) acceptor stem microhelix at 1.2A resolution. The local geometric parameters of the microhelix and the water network surrounding the RNA are presented. The structure complements the previously published Escherichia coli tRNA(Gly) aminoacyl stem structure.

PubMed: 18279665
DOI: 10.1016/J.BBRC.2008.01.175

Experimental method

X-RAY DIFFRACTION (1.2 Å)