2VUP
Crystal structure of a type II tryparedoxin-dependant peroxidase from Trypanosoma brucei
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-12-17 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.260, 32.700, 58.310 |
| Unit cell angles | 90.00, 95.92, 90.00 |
Refinement procedure
| Resolution | 58.030 - 2.100 |
| R-factor | 0.208 |
| Rwork | 0.205 |
| R-free | 0.26300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2p5q |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.114 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.000 | 2.150 |
| High resolution limit [Å] | 2.100 | 2.090 |
| Rmerge | 0.060 | 0.260 |
| Number of reflections | 9469 | |
| <I/σ(I)> | 25.5 | 12.2 |
| Completeness [%] | 96.2 | 78.5 |
| Redundancy | 3.9 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | pH 8.0 |
