2VT5

FRUCTOSE-1,6-BISPHOSPHATASE(D-FRUCTOSE-1,6-BISPHOSPHATE -1- PHOSPHOHYDROLASE) (E.C.3.1.3.11) COMPLEXED WITH A DUAL BINDING AMP SITE INHIBITOR

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	120
Detector technology	CCD
Collection date	2005-05-08
Detector	MARRESEARCH
Spacegroup name	P 1 21 1
Unit cell lengths	65.964, 284.611, 83.375
Unit cell angles	90.00, 97.52, 90.00

Refinement procedure

Resolution	30.000 - 2.200
R-factor	0.205
Rwork	0.203
R-free	0.24700
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.306
Data reduction software	XDS
Data scaling software	XSCALE
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.330
High resolution limit [Å]	2.200	2.200
Rmerge	0.060	0.290
Number of reflections	406033
<I/σ(I)>	17	4.3
Completeness [%]	87.0	46
Redundancy	3	1.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				RESERVOIR = 0.1M HEPES, PH7.0, 0.1M AMMONIUM ACETATE, 12% PEG 3350