2VSO

Crystal Structure of a Translation Initiation Complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	CCD
Collection date	2006-09-01
Detector	ADSC CCD
Spacegroup name	P 1
Unit cell lengths	69.260, 69.800, 101.970
Unit cell angles	91.70, 102.01, 115.30

Refinement procedure

Resolution	48.012 - 2.600
R-factor	0.2154
Rwork	0.214
R-free	0.26010
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ENTRIES 1QDE 1fuk 1hu3
RMSD bond length	0.008
RMSD bond angle	1.088
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.750
High resolution limit [Å]	2.600	2.600
Rmerge	0.030	0.190
Number of reflections	48104
<I/σ(I)>	14.6	3.5
Completeness [%]	93.0	91.6
Redundancy	1.8	1.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7		0.2 M TARTRATE, 20% PEG3350, PH 7.2