2VSC
Structure of the immunoglobulin-superfamily ectodomain of human CD47
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-04-12 |
Detector | MARRESEARCH |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 201.137, 47.502, 56.811 |
Unit cell angles | 90.00, 99.31, 90.00 |
Refinement procedure
Resolution | 28.190 - 1.900 |
R-factor | 0.219 |
Rwork | 0.216 |
R-free | 0.26300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2jjs CHAIN C |
RMSD bond length | 0.010 |
RMSD bond angle | 1.367 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.300 | 2.000 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.070 | 0.400 |
Number of reflections | 41496 | |
<I/σ(I)> | 13.9 | 2.9 |
Completeness [%] | 98.8 | 91.8 |
Redundancy | 3.7 | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5.5 | 200 NL 17 MG/ML CD47 PLUS 100 NL RESERVOIR (0.2 M MGCL2, 0.1 M BIS-TRIS PH 5.5, 25% W/V PEG 3350) EQUILIBRATED AGAINST 95 UL OF RESERVOIR AT 20.5 C. |