2VRI
Structure of the NSP3 X-domain of human coronavirus NL63
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X13 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-07-06 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 53.970, 54.770, 57.330 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.590 - 1.900 |
R-factor | 0.189 |
Rwork | 0.185 |
R-free | 0.24300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2acf |
RMSD bond length | 0.014 |
RMSD bond angle | 1.520 |
Data reduction software | HKL (SUITE) |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.3.0037) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 1.740 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.100 | 0.950 |
Number of reflections | 19914 | |
<I/σ(I)> | 8.7 | 1.64 |
Completeness [%] | 99.0 | 88.3 |
Redundancy | 5.5 | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | pH 7.5 |