2ACF

NMR STRUCTURE OF SARS-COV NON-STRUCTURAL PROTEIN NSP3A (SARS1) FROM SARS CORONAVIRUS

Summary for 2ACF

• Entry DOI: 10.2210/pdb2acf/pdb
• Descriptor: Replicase polyprotein 1ab, GLYCEROL (3 entities in total)
• Functional Keywords: adrp domain, sars nsp-3, appr-1-p phosphatase, structural genomics, joint center for structural genomics, jcsg, protein structure initiative, psi-2, viral protein
• Biological source: SARS coronavirus Tor2
• Total number of polymer chains: 4
• Total formula weight: 79871.84

Authors

Saikatendu, K.S.,Joseph, J.S.,Subramanian, V.,Neuman, B.W.,Buchmeier, M.J.,Stevens, R.C.,Kuhn, P.,Joint Center for Structural Genomics (JCSG) (deposition date: 2005-07-18, release date: 2006-02-14, Last modification date: 2024-02-14)

Primary citation

Saikatendu, K.S.,Joseph, J.S.,Subramanian, V.,Clayton, T.,Griffith, M.,Moy, K.,Velasquez, J.,Neuman, B.W.,Buchmeier, M.J.,Stevens, R.C.,Kuhn, P.
Structural basis of severe acute respiratory syndrome coronavirus ADP-ribose-1''-phosphate dephosphorylation by a conserved domain of nsP3.
Structure, 13:1665-1675, 2005

PubMed Abstract: The crystal structure of a conserved domain of nonstructural protein 3 (nsP3) from severe acute respiratory syndrome coronavirus (SARS-CoV) has been solved by single-wavelength anomalous dispersion to 1.4 A resolution. The structure of this "X" domain, seen in many single-stranded RNA viruses, reveals a three-layered alpha/beta/alpha core with a macro-H2A-like fold. The putative active site is a solvent-exposed cleft that is conserved in its three structural homologs, yeast Ymx7, Archeoglobus fulgidus AF1521, and Er58 from E. coli. Its sequence is similar to yeast YBR022W (also known as Poa1P), a known phosphatase that acts on ADP-ribose-1''-phosphate (Appr-1''-p). The SARS nsP3 domain readily removes the 1'' phosphate group from Appr-1''-p in in vitro assays, confirming its phosphatase activity. Sequence and structure comparison of all known macro-H2A domains combined with available functional data suggests that proteins of this superfamily form an emerging group of nucleotide phosphatases that dephosphorylate Appr-1''-p.

PubMed: 16271890
DOI: 10.1016/j.str.2005.07.022

Experimental method

X-RAY DIFFRACTION (1.4 Å)