2VR7
Crystal Structure of G85R ALS mutant of Human Cu,Zn Superoxide Dismutase (CuZnSOD) at 1.58 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX14.1 |
| Synchrotron site | SRS |
| Beamline | PX14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-01-25 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 36.768, 56.435, 75.028 |
| Unit cell angles | 90.00, 102.98, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.580 |
| R-factor | 0.137 |
| Rwork | 0.135 |
| R-free | 0.17500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2c9s |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.671 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.9999) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.640 |
| High resolution limit [Å] | 1.580 | 1.580 |
| Rmerge | 0.050 | 0.090 |
| Number of reflections | 38696 | |
| <I/σ(I)> | 20 | 8.2 |
| Completeness [%] | 93.5 | 73.5 |
| Redundancy | 3.9 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 20% PEG3350, 0.2M SODIUM THIOCYANATE, pH 7.0 |
