2VQZ

Structure of the cap-binding domain of influenza virus polymerase subunit PB2 with bound m7GTP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-1
Synchrotron site	ESRF
Beamline	ID14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2007-07-08
Detector	ADSC CCD
Spacegroup name	C 2 2 21
Unit cell lengths	92.200, 94.440, 220.400
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.530 - 2.300
R-factor	0.189
Rwork	0.186
R-free	0.23500
Structure solution method	SAD
Starting model (for MR)	NONE
RMSD bond length	0.013
RMSD bond angle	1.519
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	autoSHARP
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.080	0.480
Number of reflections	40785
<I/σ(I)>	8.59	1.79
Completeness [%]	98.4	97.3
Redundancy	3.48	3.38

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		4.6		1 MICROLITRE OF PROTEIN SOLUTION AT 11 MG PER ML IN 50 MM TRIS-HCL (PH 8.0), 200 MM NACL, 2 MM DTT, 5 MM M7GTP WITH AN EQUAL VOLUME OF A SOLUTION CONTAINING 0.1 M CITRIC ACID PH 4.6, 1.6-1.8 M SODIUM FORMATE.