2VPB
Decoding of methylated histone H3 tail by the Pygo-BCL9 Wnt signaling complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-06-16 |
| Detector | ADSC CCD |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 32.100, 79.470, 91.830 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 60.080 - 1.590 |
| R-factor | 0.194 |
| Rwork | 0.192 |
| R-free | 0.22500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vp7 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.147 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.3.0037) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.560 | 1.680 |
| High resolution limit [Å] | 1.590 | 1.590 |
| Rmerge | 0.060 | 0.370 |
| Number of reflections | 15426 | |
| <I/σ(I)> | 26.6 | 11.4 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 10.5 | 10.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 292 | HANGING-DROP VAPOUR-DIFFUSION METHOD AT 19 CELSIUS. CRYSTALLIZATION CONDITIONS: 1M NA-CITRATE, 100MM TRIS PH7, 200MM NACL, pH 8 |
