2VNK

X-RAY STRUCTURE OF THE FERREDOXIN-NADP(H) REDUCTASE FROM RHODOBACTER CAPSULATUS IN COMPLEX WITH NADP. FORM III AT 1.93 ANGSTROMS RESOLUTION

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Temperature [K]	100
Detector technology	CCD
Collection date	2005-06-10
Detector	ADSC CCD
Spacegroup name	P 1 21 1
Unit cell lengths	69.382, 93.446, 104.936
Unit cell angles	90.00, 89.97, 90.00

Refinement procedure

Resolution	49.210 - 1.930
R-factor	0.20008
Rwork	0.200
R-free	0.22615
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2bgi
RMSD bond length	0.011
RMSD bond angle	1.600
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.210	2.120
High resolution limit [Å]	1.930	1.930
Rmerge	0.080	0.220
Number of reflections	122712
<I/σ(I)>	11.5	5.2
Completeness [%]	94.6	89
Redundancy	3.4	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1