2VNF
MOLECULAR BASIS OF HISTONE H3K4ME3 RECOGNITION BY ING4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-09-12 |
| Detector | ADSC CCD |
| Spacegroup name | P 43 |
| Unit cell lengths | 68.516, 68.516, 28.174 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 68.510 - 1.760 |
| R-factor | 0.16 |
| Rwork | 0.156 |
| R-free | 0.22700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2g6q |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.816 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 68.510 | 1.810 |
| High resolution limit [Å] | 1.760 | 1.760 |
| Rmerge | 0.080 | 0.380 |
| Number of reflections | 12178 | |
| <I/σ(I)> | 9.9 | 1.7 |
| Completeness [%] | 96.9 | 86.8 |
| Redundancy | 3.8 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
