2VMY
Crystal structure of F351GbsSHMT in complex with Gly and FTHF
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.382, 103.483, 61.959 |
| Unit cell angles | 90.00, 90.33, 90.00 |
Refinement procedure
| Resolution | 20.810 - 2.700 |
| R-factor | 0.218 |
| Rwork | 0.215 |
| R-free | 0.26300 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1kl2 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.985 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.090 | 0.510 |
| Number of reflections | 19879 | |
| <I/σ(I)> | 10 | 2.4 |
| Completeness [%] | 98.7 | 99.8 |
| Redundancy | 3.5 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 50% MPD, 0.1 M HEPES PH 7.5, 0.2 MM EDTA, 5 MM 2-MERCAPTOETHANOL, 10 MM GLY, PROTEIN INCUBATED WITH 2 MM FTHF |
