2VM9
Native structure of the recombinant discoidin II of Dictyostelium discoideum at 1.75 angstrom
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-08-23 |
| Detector | ADSC CCD |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 81.833, 81.833, 257.823 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.000 - 1.750 |
| R-factor | 0.151 |
| Rwork | 0.150 |
| R-free | 0.17800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | MERCURY STRUCTURE |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.492 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.000 | 1.840 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.080 | 0.350 |
| Number of reflections | 34043 | |
| <I/σ(I)> | 7.5 | 2.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 10.9 | 11 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 200 MM AMMONIUM PHOSPHATE MONOBASIC, 100 MM TRIS PH 8.5, 50 % MPD |
