2VKV

TetR (BD) variant L17G with reverse phenotype

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2007-01-18
Detector	MARRESEARCH
Spacegroup name	P 21 21 2
Unit cell lengths	70.650, 54.460, 56.850
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.660 - 1.740
R-factor	0.18
Rwork	0.175
R-free	0.22500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2tct
RMSD bond length	0.010
RMSD bond angle	1.116
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.660	1.800
High resolution limit [Å]	1.740	1.740
Rmerge	0.060	0.380
Number of reflections	22843
<I/σ(I)>	26.2	3.8
Completeness [%]	98.6	96.9
Redundancy	10.5	8.84

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		8.7	277	0.1 M TRIS-HCL (PH 8.7), 0.3 M MGCL2, 20 % PEG 4000, 3 % DIOXANE, AT 277 K