2VKE
Tet repressor class D complexed with cobalt and tetracycline
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2007-08-01 |
| Detector | SATURN |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 67.014, 67.014, 180.806 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 62.870 - 1.620 |
| R-factor | 0.247 |
| Rwork | 0.245 |
| R-free | 0.29700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2fj1 |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.783 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 62.000 | 1.680 |
| High resolution limit [Å] | 1.620 | 1.620 |
| Rmerge | 0.080 | 0.650 |
| Number of reflections | 21642 | |
| <I/σ(I)> | 12.8 | 0.8 |
| Completeness [%] | 85.4 | 19 |
| Redundancy | 7 | 1.26 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | HANGING DROP VAPOR DIFFUSION WITH 2 UL (18 MG/ML PROTEIN) AND 2 UL (0.8 M AS, 50 MM TRIS/CL PH 7.5, 200 MM NACL, 5 MM COCL2). CRYSTAL WAS CRYOCOOLED WITH PARAFFIN OIL AS CRYOPROTECTANT. CRYSTAL SIZE WAS 0.6 X 0.6 X 0.2 MM |
