2VK0
Crystal structure form ultalente insulin microcrystals
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Spacegroup name | H 3 |
| Unit cell lengths | 81.030, 81.030, 33.900 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.520 - 2.200 |
| R-factor | 0.242 |
| Rwork | 0.240 |
| R-free | 0.28200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | MSO |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.970 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.3.0040) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.100 | 2.300 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.070 | 0.210 |
| Number of reflections | 13231 | |
| <I/σ(I)> | 11.8 | 5.7 |
| Completeness [%] | 98.8 | 98.7 |
| Redundancy | 3.3 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | pH 5.5 |
