2VJK
Formyl-CoA transferase with aspartyl-CoA thioester intermediate derived from oxalyl-CoA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-03-04 |
| Detector | ADSC CCD |
| Spacegroup name | I 4 |
| Unit cell lengths | 151.906, 151.906, 99.504 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.970 |
| R-factor | 0.175 |
| Rwork | 0.173 |
| R-free | 0.21400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1p5h |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.116 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.080 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.110 | 0.300 |
| Number of reflections | 75655 | |
| <I/σ(I)> | 10.1 | 2.4 |
| Completeness [%] | 99.2 | 97.2 |
| Redundancy | 3.6 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
