2VIS
INFLUENZA VIRUS HEMAGGLUTININ, (ESCAPE) MUTANT WITH THR 131 REPLACED BY ILE, COMPLEXED WITH A NEUTRALIZING ANTIBODY
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.85, 1.2 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 85.500, 85.500, 515.000 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 7.000 - 3.250 |
| R-factor | 0.188 |
| Rwork | 0.188 |
| R-free | 0.26700 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 27.400 * |
| Data reduction software | HKL |
| Phasing software | X-PLOR (3.84) |
| Refinement software | X-PLOR (3.84) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 3.250 |
| Rmerge | 0.109 |
| Total number of observations | 85445 * |
| Number of reflections | 18144 |
| Completeness [%] | 96.4 |
| Redundancy | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG6000 | 4 (%) | |
| 10 | 1 | drop | 0.5 (mM) | ||
| 11 | 1 | drop | 0.05 (%) | ||
| 2 | 1 | reservoir | MES | 50 (mM) | |
| 3 | 1 | reservoir | 150 (mM) | ||
| 4 | 1 | reservoir | 1 (mM) | ||
| 5 | 1 | reservoir | 0.05 (%) | ||
| 6 | 1 | drop | protein | 5 (mg/ml) | |
| 7 | 1 | drop | PEG6000 | 2 (%) | |
| 8 | 1 | drop | MES | 25 (mM) | |
| 9 | 1 | drop | 75 (mM) |
