2VG8
Characterization and engineering of the bifunctional N- and O- glucosyltransferase involved in xenobiotic metabolism in plants
Replaces: 2VCUExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Collection date | 2006-05-20 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.642, 94.418, 56.676 |
| Unit cell angles | 90.00, 110.35, 90.00 |
Refinement procedure
| Resolution | 52.490 - 1.750 |
| R-factor | 0.173 |
| Rwork | 0.171 |
| R-free | 0.20900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vch |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.615 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.3.0037) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.810 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.090 | 0.450 |
| Number of reflections | 47687 | |
| <I/σ(I)> | 14.7 | 4.25 |
| Completeness [%] | 97.7 | 100 |
| Redundancy | 3.6 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 29% (W/V) PEG 3350, 0.1M TRIS PH 8.0 |
