2VFZ
CRYSTAL STRUCTURE OF ALPHA-1,3 GALACTOSYLTRANSFERASE (R365K) IN COMPLEX WITH UDP-2F-GALACTOSE
Replaces: 2JCFExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX14.1 |
| Synchrotron site | SRS |
| Beamline | PX14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-12-07 |
| Detector | ADSC CCD |
| Wavelength(s) | 0.977, 1.48 |
| Spacegroup name | P 1 |
| Unit cell lengths | 44.735, 64.759, 66.468 |
| Unit cell angles | 83.34, 83.95, 70.14 |
Refinement procedure
| Resolution | 25.060 - 2.400 |
| R-factor | 0.178 |
| Rwork | 0.178 |
| R-free | 0.23300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.500 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.050 | 0.130 |
| Number of reflections | 27634 | |
| <I/σ(I)> | 11.5 | 4.44 |
| Completeness [%] | 89.9 | 67.2 |
| Redundancy | 4 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 10% PEG 6000, 0.1M TRIS-HCL, PH 8.0 AND 12-20% MPD |
