2VFH
Crystal structure of the F96W mutant of Plasmodium falciparum triosephosphate isomerase complexed with 3-phosphoglycerate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-02-20 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.543, 51.182, 86.568 |
| Unit cell angles | 90.00, 92.40, 90.00 |
Refinement procedure
| Resolution | 44.070 - 2.000 |
| R-factor | 0.19604 |
| Rwork | 0.193 |
| R-free | 0.24480 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1o5x |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.092 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CCP4 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.080 | 0.420 |
| Number of reflections | 31777 | |
| <I/σ(I)> | 13.5 | 2.4 |
| Completeness [%] | 99.6 | 98.7 |
| Redundancy | 3 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5 | 0.1 M SODIUM ACETATE, PH 4.0-5.5, PEG 1450 8-24% |
