2VDW
Guanosine N7 methyl-transferase sub-complex (D1-D12) of the vaccinia virus mRNA capping enzyme
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-09-20 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 |
| Unit cell lengths | 60.890, 61.020, 225.780 |
| Unit cell angles | 94.22, 92.95, 108.26 |
Refinement procedure
| Resolution | 48.390 - 2.700 |
| R-factor | 0.225 |
| Rwork | 0.223 |
| R-free | 0.26500 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.258 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.400 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.090 | 0.490 |
| Number of reflections | 75699 | |
| <I/σ(I)> | 7.31 | 1.94 |
| Completeness [%] | 89.7 | 79.6 |
| Redundancy | 1.87 | 1.83 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 10% PEG 4000, 0.1 M HEPES PH 6.5, 0.2 M LITHIUM SULPHATE, 0.3 M AMMONIUM SULPHATE, 5MM ADOHCY, 5MM GDP |
