2VDV

Structure of trm8, m7G methylation enzyme

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Temperature [K]	100
Detector technology	CCD
Detector	ADSC CCD
Spacegroup name	C 1 2 1
Unit cell lengths	128.318, 58.686, 94.760
Unit cell angles	90.00, 130.13, 90.00

Refinement procedure

Resolution	20.000 - 2.300
Rwork	0.233
R-free	0.26540
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	TRM8 FROM TRM8-TRM82 COMPLEX
Phasing software	MOLREP
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.400
High resolution limit [Å]	2.300	2.300
Rmerge	0.080	0.250
Number of reflections	22957
<I/σ(I)>	18.7	5.1
Completeness [%]	95.5	75.8
Redundancy	6.8	4.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1