2VDU

Structure of trm8-trm82, THE YEAST TRNA m7G methylation complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Detector	ADSC CCD
Spacegroup name	P 1 21 1
Unit cell lengths	53.376, 107.676, 127.749
Unit cell angles	90.00, 91.53, 90.00

Refinement procedure

Resolution	20.000 - 2.400
Rwork	0.228
R-free	0.27640
Structure solution method	SAD
Starting model (for MR)	NONE
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SHARP
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.400
High resolution limit [Å]	2.400	2.300
Rmerge	0.080	0.690
Number of reflections	26954
<I/σ(I)>	12.2	1.5
Completeness [%]	98.2	99.1
Redundancy	3.7	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1