2VDE
Crystal Structure of the Open State of TolC Outer Membrane Component of Mutlidrug Efflux Pumps
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-02-10 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 128.284, 136.176, 136.133 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 22.080 - 3.200 |
| R-factor | 0.268 |
| Rwork | 0.266 |
| R-free | 0.31500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ek9 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.033 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.100 | 3.300 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.150 | 0.420 |
| Number of reflections | 36034 | |
| <I/σ(I)> | 9.53 | 2.45 |
| Completeness [%] | 97.7 | 98.3 |
| Redundancy | 5 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 10.5 | PROTEIN WAS CRYSTALLIZED FROM 0.1M CAPS PH10.5, 30% PEG400 |
