2VCE
Characterization and engineering of the bifunctional N- and O- glucosyltransferase involved in xenobiotic metabolism in plants
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-1 |
Synchrotron site | ESRF |
Beamline | ID14-1 |
Temperature [K] | 120 |
Detector technology | CCD |
Collection date | 2006-05-20 |
Detector | ADSC CCD |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 47.807, 94.335, 57.059 |
Unit cell angles | 90.00, 110.31, 90.00 |
Refinement procedure
Resolution | 19.670 - 1.900 |
R-factor | 0.16 |
Rwork | 0.158 |
R-free | 0.19800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2vch |
RMSD bond length | 0.018 |
RMSD bond angle | 1.647 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.3.0037) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.670 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.080 | 0.370 |
Number of reflections | 37280 | |
<I/σ(I)> | 18.8 | 4 |
Completeness [%] | 99.5 | 99.5 |
Redundancy | 2.9 | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 25 % (W/V) PEG 3350, 0.1 M TRIS PH 8.5 |