2VBI
Holostructure of pyruvate decarboxylase from Acetobacter pasteurianus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-2 |
| Synchrotron site | MAX II |
| Beamline | I911-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-10-19 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 179.619, 162.434, 169.331 |
| Unit cell angles | 90.00, 102.71, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.750 |
| R-factor | 0.225 |
| Rwork | 0.224 |
| R-free | 0.24300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1zpd |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.095 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.440 | 2.900 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Rmerge | 0.080 | 0.430 |
| Number of reflections | 119238 | |
| <I/σ(I)> | 10.8 | 2 |
| Completeness [%] | 97.2 | 98.5 |
| Redundancy | 2.4 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
