2VAN

Nucleotidyl Transfer Mechanism of Mismatched dNTP Incorporation by DNA Polymerase b by Structural and Kinetic Analyses

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	100
Detector technology	IMAGE PLATE
Detector	RIGAKU IMAGE PLATE
Spacegroup name	P 21 21 2
Unit cell lengths	62.862, 119.430, 38.013
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.910 - 2.100
R-factor	0.217
Rwork	0.217
R-free	0.24900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1bpd
RMSD bond length	0.005
RMSD bond angle	1.200
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	20.000
High resolution limit [Å]	2.100	2.000
Rmerge	0.040	0.160
Number of reflections	16773
<I/σ(I)>	27.8	6.4
Completeness [%]	98.0	89.8
Redundancy	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1