2V9J

Crystal structure of the regulatory fragment of mammalian AMPK in complexes with Mg.ATP-AMP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Temperature [K]	100
Detector technology	AREA DETECTOR
Detector	MULTIWIRE
Spacegroup name	P 21 21 21
Unit cell lengths	48.790, 120.685, 127.069
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.530
Rwork	0.225
R-free	0.26600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2oox
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.530
Rmerge	0.070
Number of reflections	25861
<I/σ(I)>	10.3
Completeness [%]	96.2
Redundancy	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1