2V8W
Crystallographic and mass spectrometric characterisation of eIF4E with N7-cap derivatives
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 37.993, 99.610, 135.331 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.340 - 2.300 |
| R-factor | 0.188 |
| Rwork | 0.184 |
| R-free | 0.24300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ejh |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.727 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.300 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.160 | 0.570 |
| Number of reflections | 22664 | |
| <I/σ(I)> | 2 | 1 |
| Completeness [%] | 96.0 | 89.5 |
| Redundancy | 5 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | pH 7 |
