2V8W
Crystallographic and mass spectrometric characterisation of eIF4E with N7-cap derivatives
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC CCD |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 37.993, 99.610, 135.331 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.340 - 2.300 |
R-factor | 0.188 |
Rwork | 0.184 |
R-free | 0.24300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ejh |
RMSD bond length | 0.017 |
RMSD bond angle | 1.727 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 34.300 | 2.420 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.160 | 0.570 |
Number of reflections | 22664 | |
<I/σ(I)> | 2 | 1 |
Completeness [%] | 96.0 | 89.5 |
Redundancy | 5 | 4.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | pH 7 |