2V8N
Wild-type Structure of Lactose Permease
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.1 |
Synchrotron site | ALS |
Beamline | 5.0.1 |
Temperature [K] | 120 |
Detector technology | CCD |
Detector | ADSC CCD |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 101.370, 127.400, 188.460 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 3.600 |
R-factor | 0.294 |
Rwork | 0.294 |
R-free | 0.33300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1pv7 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.700 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 3.730 |
High resolution limit [Å] | 3.600 | 3.600 |
Rmerge | 0.120 | 0.490 |
Number of reflections | 24578 | |
Completeness [%] | 85.8 | 75.6 |
Redundancy | 2.5 | 1.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8 | pH 8.0 |