2V8F
Mouse Profilin IIa in complex with a double repeat from the FH1 domain of mDia1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 38.380, 37.270, 88.750 |
| Unit cell angles | 90.00, 98.58, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.100 |
| R-factor | 0.146 |
| R-free | 0.17300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1d1j |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | SHELX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.200 |
| High resolution limit [Å] | 1.100 | 1.100 |
| Rmerge | 0.080 | 0.400 |
| Number of reflections | 93713 | |
| <I/σ(I)> | 9.3 | 2.9 |
| Completeness [%] | 92.8 | 75.4 |
| Redundancy | 3.2 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
