2V83
Crystal structure of RAG2-PHD finger in complex with H3K4me3 peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 95 |
Detector technology | IMAGE PLATE |
Collection date | 2006-03-06 |
Detector | RIGAKU-MSC |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 54.783, 46.835, 56.963 |
Unit cell angles | 90.00, 101.46, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.400 |
R-factor | 0.1961 |
Rwork | 0.196 |
R-free | 0.23020 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PROTEIN MODEL DETERMINED BY SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.504 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.490 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.150 | 0.490 |
Number of reflections | 11048 | |
<I/σ(I)> | 8.1 | 1.8 |
Completeness [%] | 99.4 | 95.7 |
Redundancy | 3.3 | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 10% PEG 5000 MONOMETHYL ETHER, 0.1 M BIS-TRIS PH 6.5 VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 298K. | |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 10% PEG 5000 MONOMETHYL ETHER, 0.1 M BIS-TRIS PH 6.5 VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 298K. |