2V7Q
The structure of F1-ATPase inhibited by I1-60HIS, a monomeric form of the inhibitor protein, IF1.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC CCD |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 262.534, 103.271, 135.559 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.190 - 2.100 |
R-factor | 0.193 |
Rwork | 0.190 |
R-free | 0.24500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ohh |
RMSD bond length | 0.010 |
RMSD bond angle | 1.220 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | AMoRE |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 69.700 | 2.210 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.110 | 0.420 |
Number of reflections | 212416 | |
<I/σ(I)> | 9.1 | 2 |
Completeness [%] | 99.2 | 97.7 |
Redundancy | 3.5 | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8.2 | pH 8.2 |