2V62
Structure of vaccinia-related kinase 2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-05-10 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 70.655, 157.532, 56.288 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 1.700 |
R-factor | 0.203 |
Rwork | 0.201 |
R-free | 0.23600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2jii |
RMSD bond length | 0.015 |
RMSD bond angle | 1.483 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.3.0034) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.800 | 1.800 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.060 | 0.590 |
Number of reflections | 67759 | |
<I/σ(I)> | 15.3 | 2.3 |
Completeness [%] | 96.9 | 93.3 |
Redundancy | 5.8 | 5.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | 0.1 M SUCCINIC ACID PH=7.0 15% PEG3350 |