2V5T

Crystal structure of NCAM2 Ig2-3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	110
Detector technology	CCD
Collection date	2006-05-19
Detector	ADSC CCD
Spacegroup name	P 41 21 2
Unit cell lengths	114.950, 114.950, 46.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.000
Rwork	0.181
R-free	0.23450
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	IG2 MODULE OF NCAM2
Data reduction software	MOSFLM
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.110
High resolution limit [Å]	2.000	2.000
Rmerge	0.090	0.250
Number of reflections	21021
<I/σ(I)>	15.6	2.8
Completeness [%]	98.7	98.7
Redundancy	5.9	6.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		8.5		30% PEG-4000, 0.2 M LITHIUM SULFATE, 0.1 M TRIS HYDROCHLORIDE PH 8.5.