2V5R
Structural basis for Dscam isoform specificity
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-07-09 |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 277.781, 70.548, 72.771 |
| Unit cell angles | 90.00, 105.13, 90.00 |
Refinement procedure
| Resolution | 20.000 - 3.000 |
| Rwork | 0.271 |
| R-free | 0.30300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2v5m |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.200 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.100 | 0.230 |
| Number of reflections | 53580 | |
| <I/σ(I)> | 10.9 | 1.9 |
| Completeness [%] | 86.0 | 55.5 |
| Redundancy | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 10 % PEG 8000, 1MM SPERMIDINE, 0.1 M TRISHCL PH 8.5 |
