2V5H

Controlling the storage of nitrogen as arginine: the complex of PII and acetylglutamate kinase from Synechococcus elongatus PCC 7942

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-3
Synchrotron site	ESRF
Beamline	ID14-3
Temperature [K]	100
Detector technology	CCD
Collection date	2006-03-21
Detector	ADSC CCD
Spacegroup name	P 1 21 1
Unit cell lengths	90.379, 161.027, 91.562
Unit cell angles	90.00, 106.53, 90.00

Refinement procedure

Resolution	50.000 - 2.750
R-factor	0.202
Rwork	0.200
R-free	0.23600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2jj4
RMSD bond length	0.011
RMSD bond angle	1.375
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	87.710	2.900
High resolution limit [Å]	2.750	2.750
Rmerge	0.080	0.370
Number of reflections	65178
<I/σ(I)>	6.9	2
Completeness [%]	100.0	100
Redundancy	5.1	5.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		8.5		0.1 M TRIS-HCL PH 8.5, 0.15 M SODIUM ACETATE, 20% (WT/VOL) POLYETHYLENE GLYCOL 4K, 20 MM ACETYLGLUTAMATE.
1		8.5		0.1 M TRIS-HCL PH 8.5, 0.15 M SODIUM ACETATE, 20% (WT/VOL) POLYETHYLENE GLYCOL 4K, 20 MM ACETYLGLUTAMATE.