2V4Q

Post-insertion complex of the Y-family DNA polymerase Dpo4 with M1dG containing template DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	110
Detector technology	CCD
Collection date	2008-04-12
Detector	MARRESEARCH
Spacegroup name	P 21 21 2
Unit cell lengths	92.610, 103.140, 52.010
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.720 - 2.600
R-factor	0.205
Rwork	0.205
R-free	0.25400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2bqr
RMSD bond length	0.008
RMSD bond angle	1.200
Data reduction software	XDS
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	CNS (1.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.690
High resolution limit [Å]	2.600	2.600
Rmerge	0.050	0.460
Number of reflections	15832
<I/σ(I)>	23	4.4
Completeness [%]	99.0	96.8
Redundancy	5.5	5.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.4		7.5% POLYETHYLENE GLYCOL 3350, 100 MM CALCIUM ACETATE, 25 MM TRIS HCL (PH 7.4), 100 MM SODIUM CHLORIDE, 2.5% GLYCEROL, 1 MM DGTP, 5 MM CALCIUM CHLORIDE.